Bio labs

Perform the following two labs. You will need to submit complete lab reports for both of these labs. A lab report template is provided in Microsoft Word for you to download and use, see assignment resources. A lab report will include the following elements: 

Title of the Lab
Problem
Hypothesis
Method/Experimental Design (step by step instructions or a brief summary of the steps)
Results/Data
Discussion and Analysis Questions 
Conclusion

Lab #1: Experimental Design with Radish Seeds

Materials Needed: radish seeds (or some other seed that germinates quickly such as beans), small containers for growing seeds, soil, water, etc. 

Background Information: You work for a company that sells radish seeds. Your job is it determine what makes radish seeds grow quickly. You already know that in order to help the radish seeds germinate you can place them in water overnight. 
Problem: What is the problem you are trying to answer? This needs to be specific. 
Hypothesis: Write a hypothesis in the ifthenbecause format. Again, this should be specific to your lab. 
Method:
For this lab you must come up with step-by-step directions (the method) for your experiment. The following must be included along with your directions. If you don’t know these terms, do a little research. This should be specific enough that someone else can follow your directions.

What are your independent and dependent variables?
What is your control? What are your experimental groups?
What is your method? 

Set up your experiment once you have determined what you want to test. Soak your seeds in water overnight to help them germinate faster! You should use at least 5 seeds for the control group and 5 seeds for the experimental  group. 
Note: To have a good experiment, you need to test one thing only. For example, you could test  the amount of sunlight your seeds get. Everything else would be the same – the same amount of soil, water, etc.. 
Results:
Determine what data you will collect and how you will collect it. You should have data tables in this section and collect data every day of the experiment. Your experiment should run for 5 days after the first seeds germinate. A sample data table is below. Your data table may look different. You could collect data on how many seeds are growing, the height of the plants, what they look like, etc. 

 

Day 1

Day 2

Day 3

Day 4

Day 5

Radish seeds with ___________ (control)

 

 

 

 

 

Radish seeds with ____________ (experimental group)

 

 

 

 

 

 
Discussion/Analysis: 

Create at least one graph showing your data collected over several days. 
Describe in words what happened in your experiment.
How was your experiment valid?
How was your experiment reliable?
Was there any type of correlation between your tested variables? Explain. 

Conclusion:

Answer the problem question now that you have completed your experiment.
What went wrong in your experiment? 
How could you modify your experiment to get better results? 
What other factors could you test in the future?

Lab #2: Enzyme Buster 

Problem: How will placing apple slices in different substances with different pHs affect enzyme activity in apples?

Research: When apples are exposed to oxygen they brown. This browning action is speed up by an enzyme in apples. 
pH is a measure of how acidic or basic something is. A pH of 0-6 is acidic, 7 is neutral, and 8-14 is basic. 
Hypothesis: Write a hypothesis in the ifthenbecause format. 

Materials: apple slices, small cups, vinegar, lemon juice, baking soda + water, milk of magnesia, and water
Method:

1. Obtain 5 different small cups. Label one cup with each of the following:

2. Place an equal amount of liquid in each cup. You need enough to cover about half the apple slice. You will need to mix the baking soda and water (about 1 tsp of baking soda).

3. Place the apple slice into the liquid for about 5 minutes. Remove and put aside (be sure you know which apples were in which liquids!) for about 10 15 minutes. 

4. Observe the apple slices and record your observations in the data table. If you dont notice any changes (browning or no browning), leave for longer. 

Results: Describe the appearance of the apple slices dipped in each of the solutions. You may also include pictures if you’d like. 

Solution

Apple Slice Appearance 

Vinegar

 

Lemon Juice

 

Water

 

Baking Soda

 

Milk of Magnesia

 

 

1. What was the control in our experiment? 

2. Some substances can destroy enzymes and make them inactive. From your results, what substances in this lab destroyed the enzyme activity that causes browning in apples?

3. Vinegar and lemon juice are acids. Baking soda and milk of magnesia are bases. Water is neutral. From your results, would you say that acids, bases, or neutral substances destroy enzymes in apples? Why?

1. Answer the problem question now that you have completed your experiment.

2. What, if anything, went wrong in your experiment? 

3. How could you modify your experiment to get better results? 

4. What other things could you test in the future?